Journal: The Journal of Pharmacology and Experimental Therapeutics
Article Title: AT1 Receptor Antagonism Is Proangiogenic in the Brain: BDNF a Novel Mediator
doi: 10.1124/jpet.112.197483
Figure Lengend Snippet: Candesartan induced BDNF expression is mediated through AT2 receptor. To evaluate the involvement of AT2 receptor in candesartan-induced BDNF expression, hCMECs were pretreated with PD-123319 or vehicle, followed by AngII (1 ƒM or 1 µM) in the presence or absence of candesartan (0.16 µM) (A). To further confirm the role of AT2, hCMECs were treated with CGP-42112A (0.1 µM) or vehicle for 16 hours (B). *Significantly different from control, #significantly different from AngII in the same group, $significantly different from AngII+cand in the same group; overall, P = 0.02, F=3.086, n = 4–6.
Article Snippet: The involvement of AT2 receptor was assessed using the AT2 antagonist PD-123319 (0.1 µ M) and the AT2 receptor agonist CGP-42112A (0.1 µ M), both purchased from Sigma-Aldrich.
Techniques: Expressing